Intermediate
Part:BBa_K218004:Design
Designed by: Jeremy Kubik Group: iGEM09_Calgary (2009-10-19)
Terminator followed by constitutive TetR promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The strong terminator is followed by a constitutive promoter so that the transcription of the gene upstream of the terminator is adequately insulated to not influence transcription of the gene downstream of the TetR promoter. This intermediate is constructed to allow for the construction of Part:BBa_K218006.
Source
B0015 - Registry of Standard Biological Parts
R0040 - Registry of Standard Biological Parts